Mahfut, Mahfut and Angraeni, Mauilinda (2020) Evolusi Virus Anggrek di Indonesia. In: Prosiding Seminar Nasional Biologi di Era Pandemi COVID-19, 19 September 2020, Gowa, Makassar.
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Abstract
Odontoglossum ringspot virus (ORSV) is an important orchid virus worldwide. This virus has entered and infected in Indonesia, both natural orchids and hybrid orchids. Detection indicates that the virus is found in natural forests, botanical gardens and nurseries in Java, Kalimantan, Sulawesi, Bali and Papua. The following results indicate that hybrid orchids in the nursery are more susceptible to infection with ORSV than natural orchids in natural forests and botanical gardens. ORSV was found to infect more hybrid orchids, namely in 8 nursery locations, compared to natural orchids in natural forests and botanical gardens, namely only 3 locations each. The DAS-ELISA serological test showed that the mean absorbance value of ORSV infecting hybrid orchids was higher (1.125-1.152), than natural orchids in botanical gardens and natural forests, which were 0.520-0.918 and 0.520, respectively. Phalaenopsis are suitable hosts and most susceptible to infection with ORSV with an incidence of 57%, followed by Calanthe (14%), Dendrobium (9%), as well as Bulbophylum, Calanthe, Cattleya, Oncidium, and Liparis 5% each. The coat protein gene sequence analysis showed that Indonesian ORSV isolates from natural forests and botanical gardens showed a higher similarity index value with isolates from other countries (100%), compared to Indonesian ORSV isolates from nurseries (99.3%). ORSV isolates from natural forests and botanical gardens had fewer mutations, namely 18% and 36%, respectively, compared to ORSV isolates from nurseries (45%). The results of the sequence analysis also indicate that the virus has evolved, even leading to speciation. Phylogenetic tree reconstruction shows that Indonesian ORSV is thought to have originated in Germany.
| Item Type: | Conference or Workshop Item (Paper) |
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| Subjects: | Q Science > Q Science (General) Q Science > QK Botany |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) > Prodi Biologi |
| Depositing User: | MAHFUT - - |
| Date Deposited: | 16 Nov 2020 06:06 |
| Last Modified: | 16 Nov 2020 06:06 |
| URI: | http://repository.lppm.unila.ac.id/id/eprint/25462 |
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