Husaini, Iin Pertiwi A. and Mulyani, Melza and Hariri, Muhamad Rifqi and Damayanti, Inggar (2020) IDENTIFIKASI DINI JENIS-JENIS PHYLLANTHUS SECARA IN SILICO. In: National Seminar on Biodiversity and Indonesian Tropical Ecology & International Wildlife Symposium, 20-21 September 2019, Padang.
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Abstract
Phyllanthus is one of 58 family members in the Phyllanthaceae family. This genus has the largest number of members, reaching 1200 species and as many as 32 species can be found in Indonesia. Identification of species in this genus is quite difficult to do because several species share a similar characteristics. Early identification using DNA Barcoding is expected to be able to assist species identification activities more quickly and effectively. This research was conducted to determine the character and compare DNA Barcoding primers that are good to be used in the identification of Phyllanthus species. Data of DNA Barcoding sequences derived from nucleus and plastide sequences were obtained through data mining from NCBI. The dendrogram construction was carried out using UPGMA method based on Kimura-2 parameters through MEGA X software. The four dendrograms produced showed a fairly good resolution in determining the species of Phyllanthus. The ITS and matK dendrogram showed the best determination because the grouping of species was consistent with its subgenus.
| Item Type: | Conference or Workshop Item (Paper) |
|---|---|
| Subjects: | Q Science > QK Botany |
| Divisions: | Fakultas Pertanian (FP) > Prodi Kehutanan |
| Depositing User: | inggar damayanti |
| Date Deposited: | 11 Nov 2020 05:18 |
| Last Modified: | 11 Nov 2020 05:18 |
| URI: | http://repository.lppm.unila.ac.id/id/eprint/24899 |
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