Yonata, Ade (2008) EKSPRESI FLG-2 PADA EKSTRAK EPIDERMIS MANUSIA DENGAN METODE WESTERN BLOT. PROSIDING Seminar Nasional Sains dan Teknologi II. IV-91-IV-97. ISSN 978-979-1165-74-7

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Abstract

Filaggrin-2 (Flg-2) is a novel profilaggrin-like gene which is expressed in the epidermal granular layer of mouse skin. Here we study the expression of Flg-2 in normal human epidermal extract using western blot technique. Probing with anti-human Flg-2 repeat showed some bands extending from middle to bottom, suggesting that human profilaggrin-2 is processed to yield smaller intermediates protein. Probing with anti-human Flg-2 B-domain resulted in bands of 45 kDa, 28 kDa and 25 kDa, indicating some steps of Flg-2 processing where profilaggrin-2 undergoes proteolytic processing to yield some smaller fragments containing B-domain. These results showed that Flg-2 expression in human epidermal is similar to Flg-2 expression in mouse as well as Flg-1 expression in human and mouse.

Item Type: Article
Subjects: R Medicine > R Medicine (General)
Depositing User: Ade Yonata
Date Deposited: 19 Jun 2020 03:59
Last Modified: 19 Jun 2020 03:59
URI: http://repository.lppm.unila.ac.id/id/eprint/22680

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