Setiatin, E.T. and Sajuthi, Dondin and Purwantara, Bambang and Talib, C. and Fathul, Farida and Adiati, U. and Praira, W. (2011) ANTIBODY POLYCLONAL PRODUCTION ON RABBIT ANTI-OVINE PREGNANCY-ASSOCIATED GLYCOPROTEIN (Rabbit anti-ovPAG). Journal of the Indonesian Tropical Animal Agriculture, 36 (3). pp. 159-165. ISSN 2087-8273/eISSN: 2460-6278

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Antibody Polyclonal Production on Rabbit Anti-Ovine Pregnancy-Associated Glycoprotein (Rabbit anti-ovPAG).pdf

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Abstract

ABSTRACT The aim of the study was tp produce polyclonal antibody (rabbit anti-onPAG) wich could detect PAG in the urine of pregnant ewes. Twelve rabbits were immunized against ovPG DEAE-trisHCL (DT), DEAE-NaCL 20 mM (DN2), DEAE-NaCl 40 mM (DN4), DEAE-NaCl 80mM (DN 8), DEAE-NaCl 160 mM (DN16), DEAE-NaCL 320 mM (DN 32) and DEAE-NaCl 1M (DN1) and NaCl 0,9% as a placebo. The 0,5 ml of isolate (purified from ovine cotyledon) was emulsified in equal volume with complete and incomplete Freud’s adjuvant. The mixture of each isolate and adjuvant was injected at multiple sites along the dorsal area of rabbits by subcutaneous route. Blood were collected from marginal ear vein, starting before first injection (baseline) and every 14 days. Rabbit anti-ovPAG were measured using Modifield ELISA Technique. By using Western Blot Technique, DN 32 showed the best immune response among others and also could differenciate ovPAG in the urine of pregnant ewes. It could be concluded that ovPAG DN 32 is a specific source of rabbit anti-ovPAG production. Protein of ovPAGat molecular weight 31 kDa is a pregnancy protein marker of garut sheep and could be developed as a major protein for producing antibody. Keywords : cotyledon, ELISA, ovPAG, olyclonal antibody, rabbit anti-ovPAG

Item Type: Article
Subjects: S Agriculture > SF Animal culture
Divisions: Fakultas Pertanian (FP) > Prodi Nutrisi dan Teknologi Pakan Ternak
Depositing User: FARIDA
Date Deposited: 08 Jun 2020 01:24
Last Modified: 08 Jun 2020 01:24
URI: http://repository.lppm.unila.ac.id/id/eprint/22045

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