Nurhasanah, Nurhasanah and Yuwono, Suripto D. and Herasari, Dian and Ningrum, Meitri Ayu (2020) STUDI PEMANFAATAN LIMBAH CAIR KELAPA SAWIT SEBAGAI SUBSTRAT PERTUMBUHAN MIKROBA INDIGENOUS DALAM MEMPRODUKSI BOSURFAKTAN. In: Seminar Nasional Energi dan Material Maju ke-2, 17 Februari 2020, Universitas Jambi. (Unpublished)

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Abstract

Biosurfactants are amphiphatic molecules that have surface active properties and can reduce surface tension in the space between water and oil. Because of its properties, biosurfactans widely used in agriculture, cosmetics, pharmaceuticals, detergents, hydrocarbons bioremediation and others. This research aims to study the utilization of palm oil liquid waste as an indigenous microbial growth substrate in producing biosurfactants. The methods used include hemolysis tests, determination of bacterial growth curves, optimization of biosurfactant production time, pH and salinity. Determination of biosurfactant activity is done by measuring the emulsion index, oil spreading, and drop collapse. The results showed that indigenous microbial isolates LKM D1, LKT B1, and LKM C2 can produce biosurfactants by the formation of a clear zone around the disc paper in the hemolysis test, which is 12 mm in LKM D1 isolates, 40 mm in LKT B1 isolates, and 10 mm in LKM isolates C2. Determination of growth curves showed that LKM D1 and LKT B1 isolates produced biosurfactants at the end of the stationary phase, namely the 60th hour while LKM C2 isolates at the 72nd hour. Biosurfactant production uses 10% of palm oil liquid waste in the three isolates, optimum at 108 hours. The results of determining the optimum pH and salinity of biosurfactant production using 10% of palm oil liquid waste showed that LKM D1 isolates optimum at pH 7 and salinity levels of 0.5%, LKT B1 isolate optimum at pH 8 and salinity levels of 1%, and LKM C2 isolate optimum at pH 9 and salinity level of 1%. Based on these results it can be concluded that indigenous microbes are able to utilize palm oil liquid waste as a growth substrate in producing biosurfactant. Keywords: biosurfactant, emulsion index, haemolysis, indigenous microbe, palm oil liquid waste

Item Type: Conference or Workshop Item (Speech)
Subjects: Q Science > QD Chemistry
Divisions: Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) > Prodi Kimia
Depositing User: Nurhasanah Nurhasanah Husin
Date Deposited: 30 May 2020 08:25
Last Modified: 30 May 2020 08:25
URI: http://repository.lppm.unila.ac.id/id/eprint/21157

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