Mahfut, Mahfut and Daryono, Budi Setiadi and Somowiyarjo, Susamto (2020) Resistance Response of Indonesian Native Orchids to Odontoglossum ringspot virus (ORSV) Involves Expression of the CP and rbcL Genes in Cianjur, West Java. MALAYSIAN JOURNAL OF MICROBIOLOGY, xx (x). ISSN 2231-7538 (Submitted)

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Abstract

Odontoglossum ringspot virus (ORSV) is an orchid virus that is reported to be the most widely infected and has a wide spread in the world, including Indonesia. Biotic stress problems cause a decrease in the rate of photosynthesis. This study aims to determine the resistance response of Indonesian native orchids to viruses involved in the molecular analysis of the CP (coat protein) gene of ORSV and the rbcL (ribulose-1,5-bisphosphate carboxylase) gene of chloroplasts. Infected orchid leaf isolates were collected from nursery orchid cultivation in Cianjur. Amplification of the CP and rbcL genes succeeded in obtaining amplitudes measuring ± 474 bp and ± 599 bp, respectively. The results of the sequence analysis show that there are point mutations, namely deletions, insertions and transitions. The change is thought to be an adaptation and physiological response of plants to the environment in Indonesia.

Item Type:	Article
Subjects:	Q Science > QK Botany
Divisions:	Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) > Prodi Biologi
Depositing User:	MAHFUT - -
Date Deposited:	29 May 2020 06:54
Last Modified:	29 May 2020 06:54
URI:	http://repository.lppm.unila.ac.id/id/eprint/21008

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