Mahfut, Mahfut (2020) Evolution of Indonesian Orchid Virus. MALAYSIAN JOURNAL OF MICROBIOLOGY, xx (x). ISSN 2231-7538 (Submitted)

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Abstract

Odontoglossum ringspot virus (ORSV) is an important virus infecting in the world. This virus has entered and infected orchids in Indonesia, both natural orchids and hybrid orchid. Detection result indicates that the virus is found in various locations of natural forests, botanical gardens, and nurseries in Java, Kalimantan, Sulawesi, Bali and Papua. Result showed that hybrid orchids in nurseries were more susceptible to ORSV infection compared to natural orchids in natural forests and botanical gardens. ORSV was found to infect hybrid orchids in eight nurseries, compared to natural orchids in natural forests and botanical gardens, each of which was found in only 3 location. The DAS-ELISA serological test showed higher mean value of absorbance in samples collected from hybrid orchids at nurseries (1.125-1.152), compared to samples collected from natural orchids in botanical gardens and natural forests (0.520-0.918 and 0.520, respectively). Phalaenopsis was the most suitable and the most vulnerable host to be infected with ORSV (incidence rate at 57%), followed by Calanthe (14%), Dendrobium (9%), and Bulbophylum, Cattleya, Oncidium, and Liparis with incidence rate at 5%. Sequence analysis of coat protein genes showed that Indonesian ORSV isolates from natural forests and botanical gardens had higher similarity index value with isolates from other countries (100%), compared with Indonesian ORSV isolates from nurseries (99.3%). ORSV isolates from natural forests and botanical gardens had fewer mutation rate of 18% and 36%, compared to ORSV isolates from nurseries with mutation rate of 45%. The sequence analysis also indicated that the virus has evolved, even leading to speciation. Reconstruction of the phylogenetic tree showed that Indonesian ORSV was thought to originate from Germany.

Item Type: Article
Subjects: Q Science > QK Botany
Divisions: Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) > Prodi Biologi
Depositing User: MAHFUT - -
Date Deposited: 29 May 2020 06:54
Last Modified: 29 May 2020 06:54
URI: http://repository.lppm.unila.ac.id/id/eprint/20990

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