Agustian, Joni and Hermida, Lilis (2016) Hydrolysis of Starch Enzymaticaly: Immobilised Amylases on MCF Silica. In: The 2nd International Conference on Science, Technology and Interdisciplinary Research 2016, 22-25 Agustus 2016, 7th Hotel Bandar Lampung. (Unpublished)

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Abstract

As immobilisation of amylases seems to be the most promising way to obtain more stable and reusable enzymes, a review on the enzymes supports and application of Mesoporous Cellular Foam (MCF) silica as the support are presented here. Glucoamylase and alpha-amylase had been immobilized successfully in/on nanoporous, mesoporous and macroporous supports via adsorption, covalent bonding, cross-linking, entrapment and encapsulation. Factors of temperature, immobilisation time, enzyme concentration, polymer size, pH stability and reusability were studied. In general, the immobilised glucoamylase showed better thermal and pH stability and activity than the free form. Silica nanoparticles had been used to support the alpha-amylase and glucoamylase, which produced better thermal stability and enzymatic activity over its free-form. Immobilisation of the amylases in/onto gave better results than the free enzymes where quantity of the enzymes immobilised increased with the increase of the pores size.

Item Type:	Conference or Workshop Item (Paper)
Subjects:	T Technology > TP Chemical technology
Divisions:	Fakultas Teknik (FT) > Prodi Teknik Kimia
Depositing User:	JONI AGUST
Date Deposited:	31 May 2017 02:03
Last Modified:	31 May 2017 02:03
URI:	http://repository.lppm.unila.ac.id/id/eprint/2088

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