Supono, Supono and Harpeni, Esti and Wardiyanto, Wardiyanto (2019) Identification of Vibrio sp. as a cause of white feces diseases in white shrimp Penaeus vannamei and handling with herbal ingredients in East Lampung Regency, Indonesia. AACL Bioflux, 12 (2). pp. 417-425. ISSN 1844-9166

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Abstract

Abstract. White feces disease (WFD) is one of the diseases that attack white shrimp (P. vannamei). This disease causes cultivation failure and huge loss for shrimp farmers. The cause of this disease is thought to be due to the abundance of the Vibrio bacteria population in cultivation media. This study was aimed to determine the abundance of Vibrio sp. and the presence of Vibrio sp. as a trigger for WFD in P. vannamei ponds and to study the use of several herbal ingredients in suppressing the growth of Vibrio sp. Samples were taken from secondary canals, tertiary canals, primary canals, shrimp pond waters that were not infected with WFD, shrimp pond water infected with WFD, and shrimp infected by WFD. Samples taken from those locations were then inoculated; the total population of the bacteria was calculated, identified and tested for anti-bacterial activity using several herbal products. According to the results of the study, Vibrio abundance was obtained as follows: water sample was positive of WFD by 3.5±0.9×105 CFU mL-1, shrimp intestine was positive of WFD by 4.4±0.1×105 CFU mL-1, primary canal of 3.9±2.×104 CFU mL-1, secondary canal of 1.0±0.1×105 CFU mL-1, tertiary canal 3.2±1.1×105 CFU mL-1, shrimp pond 1 of 2.2±0.3×105 CFU mL-1,shrimp pond 2 of 1.3±0.3×105 CFU mL-1,shrimp pond 3 of 5.2±1.0×104 CFU mL-1 and healthy shrimp intestine of ≤2.5±0,5×104 CFU mL-1. The type of Vibrio identified and suspected of triggering WFD disease were V. vulnificus, V. parahaemolyticus, and V. alginolyticus. Antibacterial test showed that mangrove leaf extract (Rhizophora apiculata) had the best inhibitory effect on V. parahaemolyticus (zone of inhibition of 5.61 mm), followed by ketapang leaf extract (4.9 mm zone of inhibition) and papaya leaf extract (zone of inhibition of 4.5 mm). The best concentration of mangrove leaf extract in suppressing the growth of V. parahaemolyticus was 700 mg L-1. Key words : Vibrio abundance, WFD triggers, , antibacterial activity, Vibrio parahaemolyticus, Rhizophora apiculata

Item Type: Article
Subjects: S Agriculture > SH Aquaculture. Fisheries. Angling
Divisions: Fakultas Pertanian (FP) > Prodi Budidaya Perairan
Depositing User: SUPONO
Date Deposited: 13 Apr 2020 02:02
Last Modified: 13 Apr 2020 02:02
URI: http://repository.lppm.unila.ac.id/id/eprint/19098

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