Aksah, Fahrul and Nukmal, Nismah and Rosa, Emantis (2016) THE COMPARISON OF TOXICITY PURIFIED ISOLATE OF WATER AND METHANOL EXTRACTS OF PAWDER LEAF GLIRICIDIA MACULATA ON MORTALITY SOURSOP MEALYBUG PSEUDOCOCCUS CRYPTUS. Proceeding of 3rd International Wildlife Symposium. pp. 189-196. ISSN ISBN 978-602-0860-13-8
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Abstract
Soursop production in Indonesia continues to decline from year to year. One cause is the mealybug pest (Pseudococcus cryptus). The white lice suck the young fruits soursop to dry and stunted. To control the pest, using botanical insecticides more safety than synthetic insecticides. One of the plants that can be used is Gliricidia maculata. The leaves of G. maculata contain planty of an active compound flavonoid. The purpose of the study to compare the toxicity of the purified isolates of water and methanol extracts of G. maculata leaves on mortality soursop mealybugs (P.cryptus). Extraction was done by maceration series using various organic solvents (n-hexane, dichloromethane, methanol and water). Fractionation and purification of flavonoids from polar extracts were done by Chromatography Coloum. A set of laboratory experiment was conducted by using block design. Water and Methanol extracs (WE and ME) with 5 levels concentration i.e. 0%, 0.02%, 0.04%, 0.06% and 0.08%, and 3 replications. ANOVA was conducted to obtain the means and standarrd deviations of the experimental study, and Tukey’s test at α = 5% was peformed in order to obtain the different among the expelimental groups. Analisys Probit were used for compare the efectivities the exstracts. The result indicated the was toxic to mealybug pest (P. cryptus) with LC50 72 hours water extract 0,061% and metanol extract 0,096%. Therefore water extract more toxic than metanol extract. Keywords : extract water , methanol , powder leaves (G.maculata) , soursop mealybug (P.cryptus)
| Item Type: | Article |
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| Subjects: | Q Science > QL Zoology |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) > Prodi Magister Ilmu Biologi |
| Depositing User: | Dr Emantis Rosa |
| Date Deposited: | 29 May 2017 06:40 |
| Last Modified: | 29 May 2017 06:40 |
| URI: | http://repository.lppm.unila.ac.id/id/eprint/1836 |
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