Priyambodo, Priyambodo and Handayani, Niken Satuti Nur (2016) DETECTION OF EXON 1 MUTATIONS IN THE β-GLOBIN GENE ON β-THALASSEMIA CARRIER IN YOGYAKARTA. In: International Wildlife Symposium, 18 - 19 October 2016, University of Lampung. (Unpublished)

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Thalassemia is a hereditary dissorder caused by point mutation on the globin gene. βthalassemia is the most common type in Indonesia. This study aimed to performed the molecular detection of exon 1 mutation in the β-globin gene by using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) method. DNA samples was collected during 2012 to 2013 in Yogyakarta. DNA of each individual suspected βthalassemia carriers was isolated from blood and amplified with specific DNA primer and PCR conditions. Individual suspected β-thalassemia carriers with mutant alleles showed more than two DNA bands on polyacrilamide electro phoresis analysis. PCR-SSCP resultsshowed that 17 of 21 individual suspected β-thalassemia carriers have mutation site in exon 1 β-globin gene.

Item Type: Conference or Workshop Item (Speech)
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history > QH301 Biology
Q Science > QH Natural history > QH426 Genetics
Divisions: Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) > Prodi Biologi
Depositing User: Priyambodo Priyambodo
Date Deposited: 29 May 2017 06:30
Last Modified: 29 May 2017 06:30

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