Erika, Erika and Agustrina, Rochmah and Sumardi, Sumardi and Mulyono, Mulyono (2016) OPTIMASI MEDIA PRODUKSI XILANASE DARI Bacillus sp. Jurnal Selulosa, 6 (1). pp. 19-26. ISSN 2527-6662
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2016 - Jurnal Selulosa, Vol. 6, No. 1, Juni 2016 - 19 - 26 -- Erika dan Sumardi.pdf Download (482kB) | Preview |
Abstract
Xylan is a carbon source in growth medium of extracellular xylanase producing bacteria. The purpose of this study was to get the optimum medium for the growth of Bacillus sp. in producing the xylanase. The factors consist of production time, carbon, and nitrogen source, as well as simple sugars. Addition carbon source used was delignified sugarcane bagasse, rice hulls, and corn cobs with different concentrations (0.25%; 0.5%; 0.75%; and 1% w/v) . Ammonium chloride, ammonium sulfate, and sodium nitrate with different concentrations (0.08%; 0.17%; 0.26%; and 0.35% w/v) were used as a source of nitrogen, while the simple sugar used was glucose, lactose, sucrose, and xylose. The results showed that the optimum culture media of Bacillus sp. to produce xylanase is media with 0.25% natural starch from the corn cob xylan as a carbon source, 0.26% ammonium chloride as a source of nitrogen, 0.0625 grams of sugar xylose, at pH 6, incubation temperature of 40°C, and 12 hours production time. In that media, xylanase activity was 0.2 U/mL. Keywords: agricultural waste, medium optimization, xylanase, Bacillus sp.
| Item Type: | Article |
|---|---|
| Subjects: | Q Science > Q Science (General) |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) > Prodi Magister Ilmu Biologi |
| Depositing User: | ROCHMAH AG Rochmah Agustrina |
| Date Deposited: | 29 May 2017 05:32 |
| Last Modified: | 29 May 2017 05:32 |
| URI: | http://repository.lppm.unila.ac.id/id/eprint/1760 |
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