Saito, Tsutomu and Fitriana, Yuyun and Oono, Yutaka and Satoh, Katsuya and Narumi, Issay (2014) Benomyl-tolerant mutation of Entomopathogenic Fungi Induced by Ion-Beam Irradiation. Other. Japan Atomic Energy Agency, Ibaraki, Japan.

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Entomopathogenic fungi, Isaria fumosorosea and Beauveria bassiana, are important agents to control insect pests, but the fungi could be negatively affected by conventional fungicides for plant diseases such as powdery mildew. The purpose of this study is to generate mutants highly tolerant to benomyl, a major fungicide, using carbon ion beams. We have obtained some benomyl-tolerant mutants and examined the tolerant levels to benomyl, response to other fungicides, and virulence to target insects. It has been shown that β-tubulin of fungi are involved in resistance to benzimidazole fungicides such as benomyl. In this study, in order to identify the mutation sites of β-tubulin in the benomyl-tolerant mutants, we determined the nucleotide sequences of β-tubulin locus and compared with those of the wild-type strains. In B. bassiana mutants, the mutation was identified to be an A:T to C:G transversion at position 924 in the β-tubulin gene. This mutation causes an amino acid substitution at position 198 (Glu to Ala) in the β-tubulin protein. This site might be a binding target of benomyl or adjacent to the center of benomyl interaction with the β-tubulin. On the other hand, in I. fumosorosea mutants, the β-tubulin sequences were identical to that of the wild-type, suggesting that other mechanisms might be responsible for the benomyl tolerance. These results suggest that mechanisms of benomyl tolerance were different between I. fumosorosea and B. bassiana.

Item Type: Monograph (Other)
Subjects: S Agriculture > S Agriculture (General)
Divisions: Fakultas Pertanian (FP) > Prodi Proteksi Tanaman
Depositing User: YUYUN FITRIANA
Date Deposited: 07 Jun 2017 08:42
Last Modified: 07 Jun 2017 08:42

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