Triajayanti, Ade and Utami, Nurul and Betta, Kurniawan and Suwandi, Jhons Fatriyadi Identification Of Plasmodium Falciparum Merozoit Surface Protein-1 (Pfmsp-1) Gene From Malaria Patients In Hanura Area, Lampung, Indonesia. Bioscientia Medicina, 3 (4). pp. 1-9. ISSN 2598 0580

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Abstract

Background Malaria is a major health problem not only in Indonesia, but also the world. Incidence of anti malarial drug resistance has been found, and one of the possible cause is genetic factors. The changes that occur in the Plasmodium falciparum gene cause genetic variations that lead to resistance for treatment. There is a gene with high polymorphism that can be used as a gene marker of genetic variation in Plasmodium falciparum. Objective This study aim to identification of Plasmodium falciparum merozoit surface protein-1 (PFMSP-1) gene from malaria patients in Puskesmas Hanura, Pesawaran, Lampung. Methods Descriptive method with morbidity survey approach is used in this study. There are total 23 biological material stored that had been taken in 2016 from malaria patient in Puskesmas Hanura, Pesawaran, Lampung, by consecutive sampling. Identification of genetic variance of the Plasmodium falciparum gene was performed by nested PCR at Medical Faculty Biomolecular Laboratory of Lampung University. The results of this study were processed using a computer software. Results There are 23 samples that amplified following nested PCR for Plasmodium falciparum Merozoit Surface Protein-1 (PFMSP-1) gene target. In all samples studied, there are PFMSP-1 gene with six variations base pair length range from 200 to 1000 bp. Conclusions The study has successfully amplified PFMSP-1 gene from individual’s blood sample infected by Malaria in Puskesmas Hanura, Pesawaran, Lampung. Keywords: Genetic variation, Hanura, Lampung, Plasmodium falciparum, PFMSP-1

Item Type: Article
Subjects: R Medicine > R Medicine (General)
R Medicine > RA Public aspects of medicine
R Medicine > RA Public aspects of medicine > RA0421 Public health. Hygiene. Preventive Medicine
Divisions: Fakultas Kedokteran (FK) > Prodi Pendidikan Dokter
Depositing User: Dr. dr Betta Kurniawan
Date Deposited: 28 May 2020 04:47
Last Modified: 28 May 2020 04:47
URI: http://repository.lppm.unila.ac.id/id/eprint/17106

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