Mulyani, Melza and Damayanti, Inggar and Husaini, Iin Pertiwi A. and Hariri, Muhamad Rifqi (2018) The effectivity of species identification through DNA Barcoding : Study Case from Dendrobium. In: National Seminar on Biodiversity and Indonesian Tropical Ecology & International Wildlife Symposium, 20-21 September 2019, Padang. (Submitted)

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Abstract

Dendrobium is a member of Orchidaceae. This genus is the third largest genus only after Bulbophyllum and Epidendrum, reaches 1500 species. Bogor Botanical Gardens has 500 species of orchids with 104 of them are Dendrobium. A total of 133 specimens have not been identified because morphologically, they have commonly low variations among species or even have a high degree of plasticity. The approach through DNA barcoding is expected to help species identification effectively. This research was conducted as a first step in the process of selecting the best DNA barcoding primer for species identification in Dendrobium. The DNA Barcoding sequence data namely ITS2, rbcL, matK, and psbA-trnH sequences were analyzed using MEGA X and constructed into dendrograms. Unfortunately, the trees generated from rbcL, psbA-trnH, and ITS2 showed a quite clear determination yet some species are clustered in different subgenus clad. On the other hand, the matK tree produce a very clear species determination and can be recommended to be used as a single DNA Barcoding primer for identifying the Dendrobium sp. in Bogor Botanic Gardens collection.

Item Type:	Conference or Workshop Item (Paper)
Subjects:	S Agriculture > S Agriculture (General)
Divisions:	Fakultas Pertanian (FP) > Prodi Kehutanan
Depositing User:	inggar damayanti
Date Deposited:	18 Nov 2019 09:58
Last Modified:	18 Nov 2019 09:58
URI:	http://repository.lppm.unila.ac.id/id/eprint/17002

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