Srihanto, Eko Agus and Rustiati, Elly L. and Priyambodo, Priyambodo and yulianti, yanti and Virnarenata, Elsa and Pratiwi, Dian Neli and Novianasari, Tika and Angeliya, Liza Molecular Analysis: Moving forward from RAPD Based Analysis to Sequencing Method on Captive Sumatran Elephant Diversity in Elephant Training Center Way Kambas National Park. In: International Conference on Biological Sciences, 10-11 October 2019, Yogyakarta. (Unpublished)

Text ICBS-2019-Abstract_EA Srihanto et al_ok_Rev.01.doc Download (35kB)

Abstract

Random Amplified Polymorphism DNA (RAPD) is well known Polymerase Chain Reaction (PCR)-based method that use several random primers to identify the diversity of individual. The RAPD method resulting a DNA fragment profile. The DNA fragment profile from RAPD method is very important to make a whole molecular data of captive sumatran elephant in Elephant Training Center (ETC), Way Kambas National Park (WKNP). Understanding the DNA fragment profile of captive sumatran elephant in ETC WKNP based on PCR-RAPD method by using 4 different primers has been conducted under multiple year grant of Ministry of Research and Technology Higher Education under multiple year grant of Ministry of Research and Technology Higher Education, Indonesia. The DNA was well extracted by invasive sample, the whole blood, and well performed on agarose gel electrophoresis. PCR-RAPD was done by using primer OPA 5, OPA 8, OPA x, and OPA y. There was no result showed on PCR-RAPD result. PCR-RAPD is not adequate for describing the genetic diversity of captive Sumatran elephant as the primer and optimal temperature was not obtained. Primer specificity affects the temperature optimizing process to achieve the optimal temperature for primer attachment. Thus, the PCR process can run well. As the optimal temperature cannot be reached, the PCR-RAPD process fail to show DNA. PCR and GAPDH primer were tried. The DNA amplification, followed by sequencing process through PT. Genetika Science. Sumatran elephant’s DNA is obtained with the length of 218 bp.

Item Type:	Conference or Workshop Item (Speech)
Subjects:	Q Science > Q Science (General)
Depositing User:	Mrs Elly Lestari Rustiati
Date Deposited:	14 Nov 2019 11:02
Last Modified:	14 Nov 2019 11:02
URI:	http://repository.lppm.unila.ac.id/id/eprint/16043

Actions (login required)

View Item