Hapsoro, Dwi and Setiawan, Dwi and Hamiranti, Rahmadyah and Yusnita, Yusnita (2019) PENGARUH 2-iP, BA, 2,4-D, DAN TDZ PADA EMBRIOGENESIS SOMATIK IN VITRO KOPI ROBUSTA UNGGUL LAMPUNG (EFFECTS OF 2-IP, BA, 2,4-D, AND TDZ ON IN VITRO SOMATIC EMBRYOGENESIS OF SUPERIOR ROBUSTA COFFEE CLONE OF LAMPUNG). Jurnal Agrotek Tropika, 7 (3). pp. 527-537. ISSN 2337-4993

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Abstract

In vitro somatic embryogenesis of Robusta coffee consists of four stages i.e. primary callus induction, embryogenic callus induction, somatic embryo regeneration, and plantlet regeneration. This research aimed to investigate effects of plant growth regulators on primary callus induction in somatic embryogenesis of superior Robusta coffee of Lampung cv. Komari. Leaf explants were cultured on primary callus induction medium supplemented with (mg/l): BA 1; 2-iP 1; 2-iP 1 + 2,4-D 0,5 ;2-iP 1 + 2,4-D 1 ;TDZ 1 + 2,4-D 0,5; and TDZ 1 + 2,4-D 1 as treatments. The experiment was arranged in a completely randomized design with three repicates, one culture bottles per replication, five explants per bottle. Results of the experiment showed that after 4 weeks in culture all of the treatments resuted in 100% of primary callus induction, but led to different time of callus initiation and different callus weight. The earliest callus initiation (14.7 days) was acchieved by treatment with 2-iP 1 mg/l, while the latest (16.0-16.3 days) was acchieved by treatment with TDZ + 0.5-1.0 mg/L 2,4- D and BA 1 mg/l. The highest callus fresh weight was acchieved by treatments with 2-iP + 2,4-D (96-110 mg/explant) while the lowest was with 2-iP only or BA only (24-18 mg/explant). Primary calluses developed to become embryogenic when subcultured to embryogenic callus induction medium. Upon subculture to somatic embryo regeneration medium, the embryogenic calluses developed into somatic embryos. Key Words: Coffea canephora Pierre ex Froe, embriogenic, primary callus, Komari. Embriogenesis somatik in vitro kopi Robusta terdiri dari empat tahap: induksi kalus primer, induksi kalus embriogenik, regenerasi embrio somatik, dan regenerasi planlet. Penelitian bertujuan mempelajari pengaruh zat pengatur tumbuh (ZPT) terhadap pembentukan kalus primer kopi Robusta unggul Lampung klon Komari. Eksplan potongan daun ditanam pada media induksi kalus primer dengan penambahan (mg/l): BA 1; 2-iP 1; 2-iP 1 + 2,4-D 0,5 ;2-iP 1 + 2,4-D 1 ;TDZ 1 + 2,4-D 0,5; dan TDZ 1 + 2,4-D 1 sebagai perlakuan. Percobaan menggunakan rancangan acak lengkap dengan tiga ulangan, 3 botol kultur per ulangan, 5 eksplan per botol. Hasil percobaan menunjukkan, pada 4 minggu setelah tanam, semua perlakuan dapat menginduksi kalus primer 100%, namun waktu terbentuknya kalus pertama kali dan bobot segar kalus primer berbeda-beda. Kalus primer paling cepat terbentuk pada perlakuan 2-iP (14,7 hari setelah tanam), sedangkan paling lambat (16,0-16,3) pada perlakuan TDZ + 0,5-1,0 mg/L 2,4-D dan BA 1 mg/l. Bobot segar kalus primer tertinggi didapat pada perlakuan 2-iP + 2,4-D (96-110 mg/eksplan) dan terkecil pada perlakuan 2-iP atau BA saja (24-18 mg/eksplan). Kalus primer berkembang menjadi kalus embriogenik waktu dipindah ke media induksi kalus embriogenik. Subkultur kalus embriogenik ke media regenerasi menghasilkan embrio somatik. Kata kunci: Coffea canephora Pierre ex Froe, embriogenik, kalus primer, Komari.

Item Type: Article
Subjects: S Agriculture > S Agriculture (General)
Divisions: Fakultas Pertanian (FP) > Prodi Agronomi dan Hortikultura
Depositing User: Dr. Dwi Hapsoro
Date Deposited: 12 Nov 2019 11:13
Last Modified: 12 Nov 2019 11:13
URI: http://repository.lppm.unila.ac.id/id/eprint/15884

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