Gusta, Adryade Reshi and Hapsoro, Dwi and Sa'diyah, Nyimas and Yusnita, Yusnita (2011) PENGARUH MEDIA DASAR DAN BENZIL ADENIN (BA) TERHADAP PEMBESARAN SEEDLING ANGGREK Dendrobium IN VITRO. Jurnal Agrotropika, 16 (2). pp. 76-79. ISSN 0216-7662

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Abstract

Orchid such AS Dendrobium is more commonly propagated in vitro by using tissue culture techniques. In tissue culture, the type and composition of media and plant growth regulator was very crucial. The research was aimed to study the effect of media and plant growth regulator on growth of Dendrobium seedlings in yitro and was conducted in Plant Tissue Cttlture Laboratory, Faculty of Agriculture, University of Lampung, from April 2010 until October 2010. The experiment was carried out in a completely randomized design with three replicates. Treatments were arranged as 2 x 3 factorial design with media 1/2 MS and modified 1/2 MS with high P and low N) as the first factor and three concentrations of benzyl-adenine (0, 5, and 10 mg/l) as the second factor. Each experimental unit consisted of 3 culture bottles each of which containing 2 five month-old seedlings. The result showed that both media was not significantly affected the growth of Dendrobium seedlings. Whereas, the addition of benzyl-adenine (BA) at 5 mg/l and l0 mg/l in both of mediiwas able to increase shoot number from 2.8 to 5.2-5.4 and increase leave number from 9.2 to 18.0 - 18.2. Overall, the application of benzyl-adenine decreased shoot height from 3.7 to 3.0-2.7 cm), root number (from 2.7 to 1.3-0.8), and root length (from 1.6 to 1.0-0.8 cm). Keyword ; Dendrobium invitro, seedlings growth.

Item Type: Article
Subjects: S Agriculture > S Agriculture (General)
Divisions: Fakultas Pertanian (FP) > Prodi Agronomi dan Hortikultura
Depositing User: Dr. Dwi Hapsoro
Date Deposited: 07 Nov 2019 02:56
Last Modified: 07 Nov 2019 02:56
URI: http://repository.lppm.unila.ac.id/id/eprint/15810

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