Yusnita, Yusnita and Hapsoro, Dwi (2010) Proliferation of oil palm (Elaeis guineensis Jacq.) Somatic embryos as affected by plant growth regulators. In: Seminar Tahunan MAKSI dengan Tema: Penguatan Potensi dan Nilai Tambah Industri Sawiit Nasional Menghadapi Isu Global dan Daya Saing Internasional, in Bogor 8-9 December 2010., Bogor.

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Abstract

Due to its high commercial value, existing protocols of in vitro propagation of oil palm (Elaeis guineensis Jacq.) cannot be easily accessed. We have been trying to find a protocol of in vitro propagation of oil palm. Embryogenic callus induction and somatic embryo proliferation have been achieved but still need to be optimized. This research aimed to investigate effects of (1) 2,4-D, picloram, dicamba and kinetin on induction of embryogenic callus and (2) dicamba and kinetin on proliferation of somatic embryos. Three sets of treatments were arranged to answer the objectives. In Experiment I, callus was treated with 1 mg/l 2,4-D + 0,2 mg/l kinetin, 2 mg/l 2,4-D + 0,2 mg/l kinetin, 1 mg/l 2,4-D + 0,2 mg/l picloram + 0,2 mg/l kinetin, and 2 mg/l 2,4-D + 0,2 mg/l picloram + 0,2 mg/l kinetin. In Experiment II, callus was subjected to treatments 1 mg/l dicamba, 2mg/l dicamba, 3 mg/l dicamba, 1 mg/l dicamba + 0.1 mg/l kinetin, 2 mg/l dicamba + 0.1 mg/l kinetin, and 3 mg/l dicamba + 0.1 mg/l kinetin. In Experiment III, embryogenic callus was treated with 1 mg/l dicamba, 2 mg/l dicamba, 3 mg/l dicamba, 1 mg/l dicamba + 0.2 mg/l kinetin, 2 mg/l dicamba + 0.2 mg/l kinetin, and 3 mg/l dicamba + 0.2 mg/l kinetin. All the experiments used a control treatment we previously showed to be effective for induction and proliferation of embryogenic callus. Result of the Experimen I showed that mixture of 2,4-D, picloram, and kinetin was effective for induction of embryogenic callus but less effective than the control treatment. In Experiment II, 3 mg/l dicamba was effective for induction of embryogenic callus, but less effective compared to the control treatment. In Experiment III, 1 mg/l dicamba + 0.2 mg/l kinetin was found to be as effective as the control treatment. Key Words: Eembryogenesis, dicamba, kinetin, 2,4-D, picloram.

Item Type: Conference or Workshop Item (Poster)
Subjects: S Agriculture > S Agriculture (General)
Divisions: Fakultas Pertanian (FP) > Prodi Agronomi dan Hortikultura
Depositing User: Dr. Dwi Hapsoro
Date Deposited: 07 Nov 2019 02:56
Last Modified: 07 Nov 2019 02:56
URI: http://repository.lppm.unila.ac.id/id/eprint/15806

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