Yusnita, Yusnita and Sismanto, Sismanto and Hapsoro, Dwi (2010) In Vitro PROPAGATION OF Anthurium plowmanii cv. WAVE OF LOVE AND PLANTLET ACCLIMATIZATION. In: International Seminar on Horticulture to Support Food Security 2010, June 22‐23, 2010, Bandar Lampung Indonesia.

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Abstract

An efficient in vitro plant regeneration for Anthurium plowmanii cv. Wave of Love was established. Seed derived plants growing in vitro were used as sources of explants. One node cuttings of Anthurium plowmanii cv. Wave of Love were subjected to MS (Murashige & Skoog, 1962) medium supplemented with different concentrations of benzyladenine (BA), i.e. 0; 0,5; 1,0; 1,5; 2,0; 2,5; 3,0 and 5,0 mg/l and incubated under continuous fluoresent light of approximately 2000 lux. Shoots obtained in each treatment was observed after 8 weeks, 12 weeks and 16 weeks of cultures. Results showed that addition of BA to the medium from 0.5-5 mg/l significantly promoted shoot proliferation. In addition, increasing BA concentrations from 0.5 to 1.5 mg/l increased the shoot numbers as well as shoot fresh weight, while futher increase of BA concentrations (from 2.0 to 5 mg/l) in the medium resulted in levelling off the number of shoots per explant and shoot fresh weight. Maximum number of shoots and shoot fresh weights were obtained on medium supplemented with 1.5 mg/l BA. Shoot length tended to decrease with addition of and increasing concentration of BA in the medium. When one node cuttings from BA containing medium were subjected to MS medium free of growth regulators, with or without 2 g/l activated charcoal for 6 weeks, rooted plantlets were obtained. Subsequently, when these plantlets were acclimatized using mixes of pot plant media, 100% of the shoots were successfully acclimatized in the green house. Keywords: Anthurium plowmanii, BA, in vitro, shoot proliferation, acclimatization..

Item Type: Conference or Workshop Item (Paper)
Subjects: S Agriculture > S Agriculture (General)
Divisions: Fakultas Pertanian (FP) > Prodi Agronomi dan Hortikultura
Depositing User: Dr. Dwi Hapsoro
Date Deposited: 07 Nov 2019 02:56
Last Modified: 07 Nov 2019 02:56
URI: http://repository.lppm.unila.ac.id/id/eprint/15803

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