Ramadiana, Sri and Mayang, Ronald Bunga and Hapsoro, Dwi and Yusnita, Yusnita (2010) IN VITRO SEED GERMINATION, SEEDLING GROWTH AND ACCLIMATIZATION Of Dendrobium hybrids (Orchidaceae). In: International Seminar on Horticulture to Support Food Security 2010, June 22‐23, 2010, Bandar Lampung.

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Abstract

This present study was conducted to investigate effects of basal media and peptone on in vitro seed germination, and effects of basal media and activated charcoal on in vitro seedling growth of Dendrobium hybrids (Orchidaceae). Mature Dendrobium hybrids seed pods, 3 months after pollination, were surfaced sterilized. The seeds were aseptically sowed on two basal media (Hyponex or Vacin and Went (VW), with or without addition of 2 g/l peptone). Growth of protocorms were recorded at 60 days after seed sowing. Protocorms of 2.5 month old, which were approximately 0.7-1 cm in length, were germinated on two basal media with or without 2 g/l activated charcoal (AC). All media for seed germination as well as for seedling growth were enriched with 2% sucrose, 15 % coconut water, 5 % banana homogenate and solidified with 7 g/l agar powder. Seedling growth was recorded after 4 month of cultures. Results showed that best germination media for Dendrobium seeds was VW + 2g/l peptone, followed by Hyponex + peptone. The media without peptone resulted in lowest protocorm growth. The best medium for in vitro seedling growth was Hyponex + AC followed by VW + AC, whereas VW without AC resulted in the lowest seedling growth. After 4 months of cultures, most of the seedlings were 3.5-4.5 cm in size, with 3-6 primary roots and 4-5 leaves. Furthermore, eight month-old in vitro grown Dendrobium seedlings were successfully acclimatized on shreded fern stem potting medium in shaded green house, with survival rate of 95%. Keywords: Dendrobium, seed germination, seedling growth, media, in vitro.

Item Type: Conference or Workshop Item (Paper)
Subjects: S Agriculture > S Agriculture (General)
Divisions: Fakultas Pertanian (FP) > Prodi Agronomi dan Hortikultura
Depositing User: Dr. Dwi Hapsoro
Date Deposited: 07 Nov 2019 02:56
Last Modified: 07 Nov 2019 02:56
URI: http://repository.lppm.unila.ac.id/id/eprint/15797

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