Mayang, Ronald Bunga and Hapsoro, Dwi and Yusnita, Yusnita (2011) REGENERASI IN VITRO TANAMAN TEBU (Saccharum officinarum L.): INDUKSI DAN PROLIFERASI KALUS, SERTA INDUKSI TUNAS. Jurnal Agrotropika, 16 (2). pp. 52-56. ISSN 0216-7662

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Abstract

This research is a series of activities that studies the embryogenesis for somatic clonal reproduction plant sugarcane multiplication in vitro which includes the callus induction of explant pieces rolls up the leaves, the proliferation of embriogenic callus, and shoots induction of embriogenic callus. Those of three researches were conducted in a series. This experiment were carried out in The Plant Science Laboratory, the Faculty of Agriculture, the University of Lampung, Indonesia, from Oktober 2010 until April 2011. All experiments were conducted in a completely randomized design. Treatments of Experiment I were 2,4-D concentrations (0, 1, 2, and 3 mg/l) with and without 250 mg/l of casein hydrolysate, while those of Experiment II were combinations of those two chemicals, 2,4-D concentrations (1, 2, and 3 mg/l) with and without 250 mg/l of casein hydrolysate. Treatments of Experiment III were BA concentrations (1 mg/l and 2.5 mg/l). Results of the experiments showed that the best callus induction was achieved using MS media containing 3 mg/l 2,4-D, and callus induction was not significantly affected by casein hidrolysates. Concentrations of 2,4-D did not significantly influence embryogenic callus proliferation. The best shoot proliferation was attained in MS media containing 2.5 mg/l. Keywords: Benzyladenine (BA), 2,4-D, Casein Hydrolisate, sugarcane, and culture in vitro.

Item Type: Article
Subjects: S Agriculture > S Agriculture (General)
Divisions: Fakultas Pertanian (FP) > Prodi Agronomi dan Hortikultura
Depositing User: Dr. Dwi Hapsoro
Date Deposited: 05 Nov 2019 10:57
Last Modified: 05 Nov 2019 10:57
URI: http://repository.lppm.unila.ac.id/id/eprint/15597

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