Mahfut, Mahfut and Daryono, Budi Setiadi and Indrianto, Ari and Somowiyarjo, Susamto (2019) MOLECULAR PHYLOGENY OF ORCHIDS MYCORRHIZA ISOLATED FROM NATIVE TROPICAL ORCHIDS IN INDONESIA. MALAYSIAN JOURNAL OF MICROBIOLOGY, xx (x). x-x. ISSN 2231-7538 (In Press)

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Abstract

Aims: Mycorrhiza has an important role as a biocontrol agent. Its association with Phalaenopsis amabilis was molecularly identified through rDNA-ITS sequence analysis. The aims of the study were to identify isolated molecular of orchids mycorrhiza from native tropical orchids in Indonesia, conducted as one of native orchid conservation efforts in Indonesia. Methodology and results: One group of Ceratobasidium were isolated from the root of orchid plant in Yogyakarta based on morphological and microscopical analysis. Verification analysis molecular of these isolates resulted in 600-750 bp DNA products located on the ITS1-5.8S-ITS4 region. The sequenced products showed insertion and substitution occurances, which may result in strain diversity and possible variation in severity. Reconstruction of phylogenetic trees using Maximum Parsimony and Bootstrap-1000 approach showed that Indonesian isolates have undergone speciation and have been positioned in the cluster, which are already far apart from the other isolates. Conclusion, significance and impact of study: Isolate Ceratobasidium from Yogyakarta, Indonesia successfully isolated based on identification of rDNA-ITS sequences. Results of this study were expected to become the basic information in an effort of native orchid cultivation and protection against infectious diseases in Indonesia. The study was the first to report regarding Ceratobasidium isolated from native tropical orchids in Indonesia.

Item Type:	Article
Subjects:	Q Science > QK Botany
Divisions:	Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) > Prodi Biologi
Depositing User:	MAHFUT - -
Date Deposited:	05 Nov 2019 10:51
Last Modified:	05 Nov 2019 10:51
URI:	http://repository.lppm.unila.ac.id/id/eprint/15401

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