maulina, dina and sumitro, sutiman b and amin, mohamad and lestari, sri r Identification of bioactive compounds from Mirabilis jalapa L. (Caryophyllales : Nyctaginaceae) extracts as biopesticides and their activity against the immune response of Spodoptera litura F. (Lepidoptera: Noctuidae). Journal of Biopesticides, 11 (2). pp. 89-97. ISSN 0974-39IX
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Abstract
Biopesticide are biological agents sourced from the natural products to control the pest population. Mirabilis jalapa extract is one of biopesticides that contain the repellence of insects compounds to reduce the Spodoptera litura F. insect pest. This study aims to identify the bioactive compound in the M. jalapa that potentially for the biopesticide and the effect of immune response of S. litura after exposure of M. jalapa extract. This research was conducted using Liquid Chromatography-Mass Spectrophotemeter (LC-MS) analysis to the identify the bioactive compounds from M. jalapa extract. The measured indicator of the immune response wasthe average of hemocyte and phenoloxidase (PO) enzyme analysis. The results showed that M. jalapa has 30 types of specific compounds. Three of the best compound with abundant composition of M. jalapa more than 6.00% (g/dry weight) were mirabijalone B, vulgxanthin I and miraxanthinI. The compounds were identified as potential rapellents to S. litura. Additionally, concentration of 0.2% M. jalapa extract induce the average of hemocytesof S. litura (P<0.05) as muchas23.98x106 cells/ml. The PO activity occurs after 1h treatment of M. jalapa extract in concentrations of 0.1% (309.00 IU/mg) and 2h in concentration 0.1% (592.33IU/mg), whereas the concentration of 0.8% (w/v)caused decrease in the PO activities. Therefore, M. jalapa has the potential to induce the immune mechanism, their active compound has been potentially used as a biological insecticide.
| Item Type: | Article |
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| Uncontrolled Keywords: | Biopesticide, Immune defenses, LC-MS, Mirabilis jalapa, Spodoptera litura |
| Subjects: | Q Science > QK Botany Q Science > QL Zoology Q Science > QP Physiology S Agriculture > S Agriculture (General) |
| Divisions: | Fakultas Keguruan dan Ilmu Pendidikan (FKIP) > Prodi Pendidikan Biologi |
| Depositing User: | DINA MAULINA |
| Date Deposited: | 04 Nov 2019 07:42 |
| Last Modified: | 04 Nov 2019 07:42 |
| URI: | http://repository.lppm.unila.ac.id/id/eprint/14953 |
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