Herasari, Dian and Mulyono, Mulyono and Pandiangan, Kamisah Delilawati and Rilyanti, Mita and Satria, Heri Hydrogen Bond on Conformational Change During the Movement of Lid lipMNK. In: The 10th International Conference on Green Technology 2019, 2 - 3 Oktober 2019, Malang, Indonesia. (Unpublished)

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Abstract

Manuk lipase (lipMNK) is an enzyme from Geobacillus uzunensis indigenous to Manuk Crater, Garut, West Java. The catalytic activity of lipMNK is highly dependent on conformational change from closed lid (c-lipMNK) to open lid (o-lipMNK). LipMNK is a lipase that has double lids in segments 175 - 195 and 221 - 230. One of the interactions involved in this conformational change is hydrogen bond. Observation of hydrogen bond at the movement of lid was carried out by molecular dynamics simulation with AMBER software and structural analysis using VMD 1.9.3. In this conformational change of lipMNK, there are 54 hydrogen bonds that are conserved; while 26 pairs of hydrogen bond interactions in c-lipMNK are lost and 34 new hydrogen bond interactions are formed in the o-lipMNK in the lid opening movement. Out of the interactions above, there is one pair of hydrogen bonds in c-lipMNK (ARG241-TRP234) which changes pairs in o-lipMNK (ARG241-ASP175). In the o-lipMNK Trp234 forms a hydrogen bond with Leu228, with a distance of 8.77 Ao. These residues are thought to have an important role in maintaining stability and activity of lipMNK. Keywords: lipase, lipMNK, conformational change, hydrogen bond

Item Type: Conference or Workshop Item (Poster)
Subjects: Q Science > QD Chemistry
Divisions: Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) > Prodi Kimia
Depositing User: DR DIAN HERASARI
Date Deposited: 04 Nov 2019 08:27
Last Modified: 04 Nov 2019 08:27
URI: http://repository.lppm.unila.ac.id/id/eprint/14912

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