Satria, Heri and Yandri, Yandri and Nurhasanah, Nurhasanah and Laila, Aspita and Dwi Yuwono, Suripto (2019) Utilization of Native Extracellular Cellulase from Cultivated Indigenous Actinomycetes Act-4 on Ionic Liquid Pretreated Bagasse in Simultaneous Saccharification and Fermentation (SSF) Ethanol. In: International Conference on Sustainable Biomass 2019, 15-17 October 2019, Bandar Lampung, Indonesia. (Unpublished)


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Simultaneous saccharification and fermentation (SSF) technology require an understanding of suitable substrates and the role of the enzyme for high yield and productivity. Although SSF has been investigated extensively, the detail conditions for SSF of pretreated ionic liquid bagasse have yet not been in-depth determined. In this study, ionic liquid‐pretreated bagasse was used as the substrate for the production of ethanol by SSF. Native cellulase enzymes of selected actinomycetes (Act-4) was utilized in combination with the yeast Saccharomyces cerevisiae. The effects of the loading mass of pretreated bagasse (5% to 20% w/w) and of inoculum size were investigated. Initially, the concentration of produced reduction sugar was evaluated before conducting an ethanol fermentation. The ethanol yield increased with increasing fermented available sugar on the medium that influenced by Act-4 inoculum size. The highest ethanol yield, 52% of the theoretical based on the glucose present in the bagasse, was obtained at 10% substrate concentration. A higher loading mass detected inefficient hydrolysis process because of insufficient suspension formed, whereas a lower substrate concentration, 5%, resulted in a low yield. This SSF process was an open opportunity to develop a one-pot process of lignocellulosic biomass using the identified effective component to increase productivity.

Item Type: Conference or Workshop Item (Speech)
Subjects: Q Science > QD Chemistry
Divisions: Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) > Prodi Kimia
Depositing User: Dr. Eng. Heri Satria
Date Deposited: 04 Nov 2019 08:26
Last Modified: 04 Nov 2019 08:26

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