Satria, Heri and Yandri, Yandri and Nurhasanah, Nurhasanah and Dwi Yuwono, Suripto and Herasari, Dian (2019) Extracellular Hydrolysis Enzyme Activity of Some Indigenous Actinomycetes on Pretreated Bagasse using Choline Acetate Ionic Liquid. In: Green Industry Symposium, 11-12 September 2019, Universitas Airlangga Surabaya. (Unpublished)

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Abstract

Ionic liquids (ILs) pretreatment is currently becoming attractive approach for improving the efficiency of enzymatic hydrolysis on lignocellulose. Cholinium acetate ILs that contain cholinium cation combined with carboxylic acid-based anion is not only marked as biocompatible ILs but also known as a useful catalyst to pretreat biomass which could enhance enzymatic saccharification of lignocellulosic biomass. In this study hydrolysis activities of cellulase and xylanase which produce extra-cellularly by isolated actinomycetes under the presence of ILs were investigated. ILs/biomass ratio (g/g) were set up between 0.0-3.0 for pretreatment follow by hydrolysis using celullase and xylanase that excreted when actinomycetes were grown up in biomass bagasse successively. The IL/biomass ratio were as found to be 1.0 and 1.5, which achieved cellulose and hemicellulose saccharification percentages of between 65-86% and 78-90%, respectively. Since the cellulase activities were decreased to under 60% when IL/biomass ratio were increased to 2.0, 2.5 and 3.0, in contrast, xylanase activities remain stable at the range 82-98%. This experiment demonstrates the promising bio-hydrolysis of biomass in green technology to increase the yield of the biomass saccharification process.

Item Type: Conference or Workshop Item (Speech)
Subjects: Q Science > QD Chemistry
Divisions: Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) > Prodi Kimia
Depositing User: Dr. Eng. Heri Satria
Date Deposited: 04 Nov 2019 08:26
Last Modified: 04 Nov 2019 08:26
URI: http://repository.lppm.unila.ac.id/id/eprint/14876

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