Hapsoro, Dwi and Jannah, Husna Fii Karisma and Yusnita, Yusnita (2015) MULTIPLIKASI TUNAS PISANG ‘RAJA BULU’(Musa spp. AAB) IN VITRO PADA MEDIA YANG MENGANDUNG BENZILADENIN DAN KINETIN. In: Seminar Nasional Sains dan Teknologi VI, 3 November 2015, Bandar Lampung Indonesia.

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19. Dwi Hapsoro et al 2015 Prosiding Semnr SATEK VI UNILA.pdf

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Abstract

In vitro propagation of banana cv. ‘Raja Bulu’ (Musa spp. AAB) has been achieved with little successes due to phenolic compound exudation. This research aimed to investigate effect of benziladenine (BA) and kinetin (KIN) on in vitro shoot multiplication using shoot tips from corm (Experimen I) and from in vitro shoot culture (Experiment II). The two experiments were arranged in a completely randomized designed with three replications. Each replication consisted of three 250 ml-bottles, with 4 explants per bottle. The treatments were combinations of BA concentrations (2, 4, and 6 mg/l) and KIN (0 and 2 mg/l). Media contained MS salts supplemented with 30 g/l sucrose, 100 mg/l myo-inositol, 0.1 mg/l thiamine-HCl, 0.5 mg/l pyridoxine-HCl, 0.5 mg/l nicotinic acid, 2 mg/l glycine, plant growth regulators as treatments, and agar as a solidifying agent. Results of Experiment I showed that, with or without KIN, an increase in BA concentrations from 2-4 mg/l led to an increase in shoot multiplication rate. Results of Experiment II also showed that an increase in BA concentrations also resulted in an increase in shoot multiplication. Addition of KIN resulted in an increase in shoot multiplication. Number of propagules produced was 5,83-9,78 propagules per explant. Keywords: Banana, Benzyladenine, In Vitro, Kinetin, ‘Raja Bulu’

Item Type: Conference or Workshop Item (Paper)
Subjects: S Agriculture > S Agriculture (General)
Divisions: Fakultas Pertanian (FP) > Prodi Agronomi dan Hortikultura
Depositing User: Dr. Dwi Hapsoro
Date Deposited: 04 Nov 2019 08:26
Last Modified: 04 Nov 2019 08:26
URI: http://repository.lppm.unila.ac.id/id/eprint/14859

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