Hapsoro, Dwi and Alisan, Mochamad Ivan and Ismaryati, Titiek and Yusnita, Yusnita (2010) EFFECTS OF BENZYLADENINE ON IN VITRO SHOOT MULTIPLICATION OF BANANA (Musa paradisiaca Linn) cv. AMBON KUNING AND TANDUK. In: International Seminar on Horticulture to Support Food Security 2010, 22-23 june 2010, Bandar Lampung.

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Abstract

Objective of this research was to investigate effects of benzyladenine (BA) concentrations on in vitro shoot multiplication of banana (Musa paradisiacal Linn) cv. Ambon Kuning and Tanduk. Shoot buds (0.5 x 0.5 x 0.5 cm3) as explants were surface sterilized by shaking them in Nahypochlorite solution (1.05%) added with 1-2 drops of Tween 20 for 15 minutes and shaking them again in the same solution ( 0.5%) for 5 minutes. The explants were then rinsed in sterile distilled water at least three times. The explants were aseptically cultured on semi-solid medium consisting of MS salts, 30 g/l sucrose, 0.1 mg/l thiamine-HCl, 0.5 mg/l pyridoxine-HCl, 0.5 mg/l nicotinic acid, 2 mg/l glycine, 100 mg/l myo-inositol,2 g/l gelrite as solidifying agent, and different concentrations of BA (0, 1, 2, and 5 mg/l) as treatment. One explant was cultured on one 250 ml-culture bottle containing 20 ml medium. The pH of medium was adjusted to 5.8 prior to being autoclaved at 1.2 kgf/cm2 for 15 minutes. Each treatment consisted of at least 10 culture bottles. Data was subjected to analysis of variance and differences in treatment means was detected using least significant difference (LSD). Results of the experiment showed that BA was effective to induce shoot multiplication of both cultivars of bananas. Increased BA concentrations led to increased number of shoots. Best shoot multiplication was achieved using 2 mg/l BA, resulting in 6.8 shoots per explants for cv. Ambon Kuning and 7.3 shoots per explants for cv. Tanduk. All shoots were successfully rooted and acclimatized. Keywords: banana, Musa paradisiacal Linn, benzyladenine, in vitro, shoot multiplication.

Item Type: Conference or Workshop Item (Paper)
Subjects: S Agriculture > S Agriculture (General)
Divisions: Fakultas Pertanian (FP) > Prodi Agronomi dan Hortikultura
Depositing User: Dr. Dwi Hapsoro
Date Deposited: 04 Nov 2019 08:26
Last Modified: 04 Nov 2019 08:26
URI: http://repository.lppm.unila.ac.id/id/eprint/14858

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