Kawachi, Takashi and Tanaka, Shun and Fukuda, Akinori and Sumii, Yuji and Setiawan, Andi and Kotoku, Naoyuki and Kobayashi, Motomasa and Arai, Masayoshi (2019) Target Identification of the Marine Natural Products Dictyoceratin-Aand-CasSelectiveGrowthInhibitors in Cancer Cells Adapted to Hypoxic Environments. Marine Drugs, 17 (163). pp. 1-14.

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Hypoxia-adapted cancer cells in tumors contribute to the pathological progression of cancer. The marine spongean sesquiterpene phenols dictyoceratin-A (1) and -C (2) have been shown to induce hypoxia-selective growth inhibition in cultured cancer cells and exhibit in vivo antitumor effects. These compounds inhibit the accumulation of hypoxia-inducible factor-1α (HIF-1α), which is a drug target in hypoxia-adapted cancer cells, under hypoxic conditions. However, the target molecules of compounds 1 and 2, which are responsible for decreasing HIF-1α expression under hypoxic conditions, remain unclear. In this study, we synthesized probe molecules for compounds 1 and 2 to identify their target molecules and found that both compounds bind to RNA polymerase II-associated protein 3 (RPAP3), which is a component of the R2TP/Prefoldin-like (PEDL) complex. In addition, RPAP3-knockdown cells showed a phenotype similar to that of compound-treated cells.

Item Type: Article
Subjects: Q Science > QD Chemistry
Divisions: Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) > Prodi Kimia
Depositing User: Dr. Andi Setiawan
Date Deposited: 24 May 2019 07:16
Last Modified: 24 May 2019 07:16
URI: http://repository.lppm.unila.ac.id/id/eprint/13214

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