Nurhasanah, Nurhasanah and Nurbaiti, Santi and Madayanti, Fida and Akhmaloka, Akhmaloka (2015) DIVERSITY OF GENE ENCODING THERMOSTABLE LIPASE FROM COMPOST BASED ON METAGENOME ANALYSIS. International Journal of Integrative Biology, 16 (1). pp. 7-12. ISSN 0973-8363

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Abstract

Five lipase gene have been cloned from domestic compost through metagenomic approach. Based on the sequence of the genes, all of the the clones show highly homolog to the lipase from Pseudomonas genus. The highest homology appeared to the Pseudomonas stutzeri gene with the homology varies from 96-100%. In addition, all clones showed similar motif to some conserved region of lipase family I.1. Based on the homology analysis, the clones are suspected to Pseudomonas stutzeri lipase family I.1 group. Two clones showed significant differences among the others, namely LK 3 and LK 5. LK 3 contains deletion of 31 amino acid residues in the N-terminal region resulting on lacks of the signal peptide region. While LK 5 showed a few amino acid substitution at around Asp255 as catalytic residue, in addition, Asp255 was replaced by Thr255. Further analysis on the superimposed of 3D structure prediction between LK 5 with 1ex9 as template showed that the catalytic residues (Asp255) and the surrounding region exhibited difference orientation when replaced by Thr255 and other amino acid substitution in LK 5 clone. Based on the above result suggested that amino acid substitution on LK 5 changed the conformation of the active center at the enzyme.

Item Type:	Article
Subjects:	Q Science > QD Chemistry
Divisions:	Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) > Prodi Kimia
Depositing User:	Nurhasanah Nurhasanah Husin
Date Deposited:	16 Dec 2016 01:17
Last Modified:	16 Dec 2016 01:17
URI:	http://repository.lppm.unila.ac.id/id/eprint/1318

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