Pangesti, Ika Rachma and Suharjo, Radix and Fitriana, Yuyun and Merdiana, Eryka and Pasaribu, Lita Theresia and Swibawa, I Gede and Hidayat, Kuswanta Futas (2018) Study of Talaromyces spp. with Multiple Beneficial Task. In: Inter Academia Asia 2018, The 5th Conference, 3-5 December 2018, Shizuoka Jepang. (Unpublished)

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Abstract

This study was aimed to reveal the identity of Talaromyces spp. and their ability as entomopathogen, antagonist, phosphate solubilizing fungi, chitin degrading fungi, and Plant Growth Promoting Fungi (PGPF). Six isolates of Talaromyces spp. were used in this study. Those all isolates were obtained from three different plants rhizosphere, namely pineapple (AS-2 UNILA, AS-3 UNILA, AS-4 UNILA, AS-5 UNILA), corn (AS-8 UNILA), and chili (AS-11 UNILA). Identification was performed on the basis of morphological characteristics and molecular technique using sequence analysis of Internal Transcribed Spacer (ITS) region. Ability as entomopathogen was investigated on cocoa mirid bug (Helopeltis spp.). Antagonist test was conducted using dual culture method against Phytophtora sp. which was isolated from cocoa pod rot symptom. Ability as phosphate solubilizing fungi and chitin degrading fungi was conducted on pikovskaya and chitin agar medium, respectively. Ability as PGPF was achieved using cucumber plant as indicator plant. The data collected in this study were identity of the isolates, mortality of cocoa mirid bug, percentage of inhibition againt Phytophtora sp., area of the clear (halo) zone around the colony which was grown on pikovskaya and chitin agar media, plant height, wet weight of shoot, greenish leaves and percentage of shoot N-content. Based on the morphological characteristics, it was confirmed that all the isolates were in the group of Talaromyces. Sequence analysis result of ITS region (ITS1-ITS4) revealed that all the isolates were placed in the group of Talaromyces sayulitensis. All the isolates showed capability as entomopathogen (16.67-46.67% of mortality), antagonist (56.03-74.57% of inhibition), phosphate degrading fungi (27.40-35.80 mm2 of clear (halo) zone area) and chitin degrading fungi (17.30-24.30 mm2 of clear (halo) zone area). Three isolates showed capability as PGPF (AS4-UNILA, AS8-UNILA and AS11-UNILA) but not for the other 3 isolates (AS2-UNILA, AS3-UNILA and AS5-UNILA).

Item Type: Conference or Workshop Item (Speech)
Subjects: S Agriculture > S Agriculture (General)
Divisions: Fakultas Pertanian (FP) > Prodi Proteksi Tanaman
Depositing User: YUYUN FITRIANA
Date Deposited: 02 Apr 2019 09:04
Last Modified: 02 Apr 2019 09:04
URI: http://repository.lppm.unila.ac.id/id/eprint/11064

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