Susanto, Gregorius Nugroho and Sutyarso, Sutyarso and Rustanti, Tri (2018) SEA CUCUMBER’S STEROID EXTRACT (SCSE) AS SOURCE OF TESTOSTERONE HORMONE IN GENITAL REVERSION OF JUVENILE REDCLAW CRAYFISH. In: The 8th International Conference of Aquaculture Indonesia (ICAI) 2018, 25-27 October 2018, Yogyakarta. (Submitted)

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Abstract

Sea cucumbers, belong to the class Holothuroidea, are abundant soft-bodied echinoderms found nearly in the benthic areas across sea floor environment in Lampung, Indonesia. One of the bioactive compounds contained in sea cucumber is steroid components that serves as aphrodisiac and sex reversal. In the aquaculture development, one way to increase the production is through the single gender (monosex) cultivation by directing genital reversal. This biotechnology method can be applied using natural or synthetic hormones. The synthetic hormone commonly used is 17α-methyltestosterone. It has a very high rate of success in the male formation up to 100 %, but it leaves residues that are dangerous for human health, environment, and cultivated organisms. A research needs to be done to obtain steroid hormone from the nature which is safe for consumers and environments. One approach is to exploit hormones extracted from the visceral organs of sea cucumbers (Holothuria scabra). Therefore, the aims of the research is to identify the level of testosterone in hemolymph and male formation of juvenile redclaw crayfish after exposure in the sea cucumber’s steroid extract (SCSE). The research was designed using factorial completely random design, with two variables consists of SCSE dose variations and immersion time. Treatment of SCSE in juvenile redclaw crayfish with dipping method, using four-dose treatment of 0, 2, 4 mg/L, one positive control, and dipping time of 18 and 30 h. Each treatment has three replications. The result indicated that SCSE give an effect on elevation of testosteron level and male genital formation of juvenile redclaw crayfish. There was an interaction between dose and immersion time in the SCSE on the testosterone levels. The highest testosterone levels was found in the SCSE dose of 4 mg/L after 30 h exposure at 0,248 ng/mL and the male formation was 77,45%. The interaction between dose and dipping time had a significantly effect on the growth, biomass and testosterone concentration. Keywords : Sea Cucumber, Steroid, Testosterone, Genital Reversion, Redclaw Crayfish

Item Type:	Conference or Workshop Item (Speech)
Subjects:	Q Science > QP Physiology
Divisions:	Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) > Prodi Magister Ilmu Biologi
Depositing User:	Dr. MSc. Gregorius Nugroho Susanto
Date Deposited:	16 Nov 2018 17:31
Last Modified:	16 Nov 2018 17:31
URI:	http://repository.lppm.unila.ac.id/id/eprint/10055

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