Priyambodo, Priyambodo and Rustiati, Elly L. and Pratiwi, Dian Neli and Zahrah, Lathifah Noor and Arsan, Zulfi and Ferawati, Ni Made and Nilam, Agvinta (2018) DNA Extraction for Phylogenetic Study of Badak Sumatera di Sumatran Rhino Sanctuary, Way Kambas National Park. In: Seminar Nasional dan Rapat Tahunan (SEMIRATA) Bidang MIPA BKS PTN Barat Tahun 2018, 4-6 Mei 2018, Medan International Convention Center (MICC). (Unpublished)

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Abstract

Epithelial cells from fecal material samples provide a convenient source of DNA for phylogenetic studies. This study aimed to develop a convenient method to obtain epithelial cells from fecal material samples to be used as a source of DNA, and to evaluate the stability of the fecal DNA of badak sumatera based on time series sampling activity. The sampling procedures used swab method with DETs buffer as buffer solution. DNA extraction was done by commercial procedure of QIAMStool DNA extraction kit. DNA molecules was visualized on 1% agarose gel electrophoresis by digidoc. The results showed DNA band on first and second sampling period, but missed the DNA band on third period. The missing DNA band on third sampling period caused by biodegradation of fecal material samples.

Item Type: Conference or Workshop Item (Paper)
Subjects: Q Science > Q Science (General)
Divisions: Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) > Prodi Biologi
Depositing User: Priyambodo Priyambodo
Date Deposited: 25 Jun 2018 03:43
Last Modified: 25 Jun 2018 03:43
URI: http://repository.lppm.unila.ac.id/id/eprint/7684

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