Abstract

Tobacco is one of the important plantation commodities in Indonesia. The multiple virus attack in the field causes crop damage to crop failure. Therefore, detection is needed quickly and accurately as a basis for control strategies. This study aimed to determine the viruses that attack tobacco plants in Klaten, Indonesia through simultaneous detection using multiplex-PCR. The study was conducted by observation in the field then detection in the Laboratory. Based on Multiplex-PCR detection results using primers of MJ 1/MJ 2, Krusty/Homer, and CMV P1/P2 showed that tobacco plants infected with several viruses from the genus Potyvirus, Begomovirus and Cucumovirus. Based on the results of the nucleotide sequencing blast, it was found that the tobacco isolate was positively infected by the Tomato yellow leaf curl Kanchanaburi virus (TYLCV), Cucumber mosaic virus (CMV) and Potato virus (PVY).